Author Archive

Impact of pH on Derivatization of Nitrophenol Compounds

Our goal of evaluating the Normalized French Environmental Method 12673 required testing the method’s ability to derivatize nitrophenols and alkylphenols in addition to the chlorophenols the method was designed for. Unfortunately, nitrophenols proved challenging to derivatize at low concentrations. In an effort to improve the derivatization of nitrophenols, we experimented with the pH of the […]

Improved Peak Symmetry as a Result of Derivatization of Phenols

Peak symmetry is important for determination and quantification of compounds. In order to create a symmetric peak, the compound must be able to elute all at more or less the same time. If a compound interacts with the column itself (ex: by temporarily adsorbing to the fused silica surface), it delays travel and effects peak […]

Changes in Elution Order between Phenols and Phenyl Acetates

In order to determine contamination levels for phenols in water, the French normalized method, NF EN 12673, recommends an in-situ derivatization with acetic anhydride, followed by extraction with hexanes. The derivatization process (diagrammed in Figure 1) converts the phenolic compounds into phenyl acetates, which perform much better on GC/MS columns such as the Rxi-5Sil, due […]