Archive for the ‘Tips & Tricks’ Category

A better way to configure your EZ No-Vent GC-MS Connector Part II

Last time, I wrote a blog (here) that showed a better way to configure the EZ No-Vent in the software so that the column length didn’t need to be manipulated. I kept the volumetric flow the same, and showed different ways to minimize the negative chromatographic effects of the reduced linear velocity. Today, I’m going […]

EXP Fittings: Which Nut Do I Need?

Our brilliant friends at Optimize Technologies are continually innovating and making our lab lives easier. We love their patented EXP Titanium Hybrid Ferrule technology that is truly universal. You can use it on any tubing, any 10-32-port, any instrument—wrench-tight to 20,000 psi. This fully UHPLC-capable EXP ferrule is used with all EXP nuts. Restek offers […]

A better way to configure your EZ No-Vent GC-MS Connector

If you are using the EZ No-Vent for Agilent mass spectrometers (cat.# 21323), you are probably tricking your instrument into working properly by inflating the length of your column in your acquisition software. If you have MassHunter, or a recent version of MSD ChemStation  (G1701EA), you don’t have to do this. Instead of configuring your […]

How do I Replace my LC Tubing?

For those that are experienced with LC, you probably have this all figured out.  If that is the case, this might serve as a good introduction to Restek products that you can use for this.  For those that are newbies to liquid chromatography, welcome and I hope you find this useful! The best way to […]

Can I use a 100% aqueous mobile phase with my LC column?

You might be asking this because you have read that not all columns are OK to use with highly aqueous content (>95%) in the mobile phase.  Or maybe the caption caught your eye because you have recently encountered some difficulty with using highly aqueous mobile phase. There is a phenomenon called “phase dewetting” that can […]

General (very general) guidelines to help meet GC detection limits.

Sometimes we (tech service) receive requests from customers who cannot meet detection limits when developing a new method or when trying to follow a current method. They ask for advice on how to meet these limits.  As a result, I decided to provide some (very) general guidelines that I would follow back-in-the-day when I would […]

How (Not) To Cut Your Capillary Column

There are numerous ways to cut a capillary column. Restek has several tools to accomplish this job. There are sapphire scribes, ceramic scoring wafers, wafers with a handle, and diamond blade column cutters. There are also some tools manufactured by other companies that will cut a capillary column. The choice is typically personal preference. No […]

Should I use LC or GC for my analysis?

Although most analysts already know which approach they need to use for analysis, this is occasionally a topic of discussion.  While this blog post is not meant to give an absolute answer for each specific application, I hope to provide some tips to steer you in the general direction towards a solution, if this is […]

Is the column shown on the COA for a chemical reference standard the best column for these compounds?

  In some cases the answer is “yes”. In most cases the answer is “no”.   So why would we choose a column that is not the optimal for a specific standard? It’s because of the large number of reference standards we sell. Each mix needs to have its own validated method. If we were […]

A little extra information for installing/conditioning a new packed/micropacked column

Even though we include instruction sheets with most of our packed/micropacked columns which contain enough information for most customers, some customers (many who are new to using packed columns) contact us routinely asking for some additional assistance. As a result, I decided to add some additional information to our current instruction sheets hoping it helps those […]