Does a different oven temperature programming rate change overload for PAHs on a GC column?

Jaap proposed that it might make a difference, so I wanted to test that possibility and add it to the following series on sample loading capacity, or “loadability” as Jaap calls it, for gas chromatography.

Sample loading capacity for PAHs on a 30m x 0.25mm x 0.25µm Rxi-5ms GC column

Does a thicker film GC column increase sample loading capacity for PAHs?  30m x 0.25mm x 0.50µm Rxi-5ms

Sample loading capacity for PAHs, part 3:  30m x 0.32mm x 0.25µm Rxi-5ms

Finally, some increased sample loading capacity for PAHs! Part 4: 30m x 0.32mm x 0.50µm Rxi-5ms

As previously, I used an Rxi-5ms GC column with efficiency-optimized hydrogen carrier gas flow and optimal heating rate (in this case, 10.1°C/min) and test conditions employing 0.5x and 1.5x that heating rate, 5.1 and 15.2°C/min, respectively.

The resulting chromatograms are shown below and to my eye there are no obvious practical differences in peak shapes that would suggest overload has been changed by any of the GC oven heating rates.  The chromatograms look different as regards separation though because the PAHs are eluting at different temperatures.  I’ve listed the elution temperatures for the last PAH on the chromatograms.

As always, the EZGC Method Translator and Flow Calculator was used to guide this work.

Overload Temp

 

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