HPLC columns for the European Pharmacopoeia methods

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European Pharmacopoeia (EU) methods tend to be vague in their description for column requirements, which can make choosing the correct column a little tricky.   To simply things, I listed the Restek HPLC columns that best match the columns (phases) listed in the European Pharmacopoeia “Reagents” chapter. Hopefully this makes it a little easier to choose the correct column for your particular EU method. If you have any questions, simply send them to support@restek.com or contact your local distributor.  For GC column suggestions for the EU methods, refer to our GC capillary columns for the European Pharmacopeia methods post. Thank you.

 

Nomenclature Reference Code Restek HPLC Column
Silica gel for chromatography 1076900 Roc Silica
Silica gel for chromatography, octadecylsilyl 1077500 Roc C18
Silica gel for chromatography, octdecylsilyl R1 1110100 Roc C18
Silica gel for chromatography, octadecylsilyl, base-deactivated 1077600 Roc C18
Silica gel for chromatography, octadecylsilyl, end-capped 1115400 Roc C18
Silica gel for chromatography, octylsilyl 1077700 Roc C8
Silica gel for chromatography, octylsilyl R1 1077701 Roc C8
Silica gel for chromatography, octylsilyl, base-deactivated 1131600 Roc C8
Silica gel for chromatography, octylsilyl, end-capped 1119600 Roc C8
Silica gel for chromatography, trimethylsilyl 1115500 Ultra C1
Silica gel for chromatography, aminopropylsilyl 1077000 Ultra Amino
Silica gel for chromatography, nitrile 1077300 Roc Cyano
Silica gel for chromatography, nitrile R1 1077400 Roc Cyano
Silica gel for chromatography, nitrile R2 1119500 Roc Cyano

 

8 Responses to “HPLC columns for the European Pharmacopoeia methods”

  1. Ivo Guerreiro says:

    Hi,

    I was looking for this information exactly but the list is missing a few “reagents” from the EP.
    The organosilica ones are missing.

    I was specially interested on EP’s reference code 1150600.
    Right now the only one I know that matches the code is Waters Xterra RP but I’m looking into alternatives to Waters.

    Any sugestions?

    Thank you very much,

    Ivo Fernandes

  2. Tim Herring says:

    Hello Ivo,
    Thank you for checking out my blog post.
    The EP reference code 1150600 references a material or column that Restek does not offer.
    This particular code specifies for an organosilica polymer, which is composed of hybrid particles containing both inorganic (silica) and organic (organosiloxanes) components. Restek does not offer any hybrid nor polymeric HPLC columns unfortunately. Therefore we do not have a match or equivalent to the Waters Xterra RP. Only EP “reagents” that Restek can match are included in this post.
    Good luck in your analytical endeavors Ivo.
    Tim

  3. Nguyễn Văn Giới says:

    refrence Code: 1153500 is equivalent which the column? I need more information that column? Thank you!

  4. Tim Herring says:

    Thank you for inquiring.
    Unfortunately however Restek does not offer carbon-based HPLC columns, which is what EU code 1153500 calls for.
    I am sorry we could not help.

  5. Rudolf says:

    Hi,

    I am looking for the column from the PH.EU for HPLC of levodopa. “spherical di-isobuyloctadecylsilyl silica gel column for chromatography R (5 micro meter) with a porze size of 8 nm”. (I think ref. number is 1140000). Can you help me to find this column?

    Kind Regards,
    Rudolf

  6. Tim Herring says:

    Hello Rudolph,

    The Raptor ARC-18 would most closely match this description.
    Please refer to the “description” tab within the product information page contained in the link below.
    http://www.restek.com/catalog/view/41315

    Tim

  7. Rakesh says:

    hello sir

    i just wanted to know the difference between silica bonded column and nitrile bonded column and whether a silica bonded column can be used instead of a nitrile column.

  8. Tim Herring says:

    Hi there. A silica-based column can have many stationary phases. C18, cyano (nitrile), C8, biphenyl are all examples of different stationary phases, which can be bonded to the surface of the silica.

    So to answer your question, a nitrile bonded column IS a specific silica bonded column.

    I hope that helps.

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