Posts Tagged ‘HPLC’

Upcoming Restek Cannabis Testing Seminar

10/9 UPDATE- This seminar has been cancelled. Stay tuned for future dates.  New to chromatography? Looking to brush up on your current analytical skills? Interested in what different types of cannabis testing currently exist? The analytical market for cannabis is a rapidly growing, burgeoning field. As more and more states legalize cannabis, an increased number […]

What can I use to Qualify my HPLC instrumentation?

If you are setting up LC instrumentation in a new lab, you may find yourself looking for ways to perform Operational Qualification (OQ) tests. The requirements for qualifying a new instrument vary, depending on regulatory needs and SOP requirements, as well as the specific instrumentation. They might involve tests to confirm parameters such as solvent […]

How can I use Raptor columns and the EXP Direct connect holder with stainless steel fittings?

Some customers may find themselves with this scenario. You have an HPLC instrument with existing stainless steel tubing and fittings and you are getting ready to install a Raptor column. The end of your tubing may very well look like the one shown above. Here are some questions you might have.   Can I attach […]

LC Columns: High-Pressure Survival of the Fittest!

As an R&D Chemist charged with developing the perfect packing method for new LC columns, one of the challenges is finding a packing method that results in a column which not only passes QC specifications but also passes our arduous lifetime analysis. During lifetime testing our columns are subjected to 1,000 continuous injections at 12,000 […]

The Clog Blog

Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen […]

Need parts for your Agilent 1100? HP 1050? Restek has you covered.

As of May 31, 2015, 1100 Series HPLC systems were categorized “End of Guaranteed Support” (EGS) by Agilent. Agilent would prefer it if you purchased a new system from them, but 1100 HPLCs are dependable workhorses. Why not keep them running with instrument replacement parts from Restek? Check out our selection, easily sorted by instrument and module: […]

Which guard cartridges and holders go with which LC analytical columns?

As our family of LC products has grown, so have our choices for columns and accompanying guard cartridges. Here is a handy chart to show which guard holder and guard cartridges were intended for use with each type of analytical column. Please feel free to click the links to access each group of products on […]

What can I use to filter my mobile phase?

If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed […]

What is the pressure limit for my LC column?

This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ROC LC columns, the maximum operating pressure is 400 […]

Is it OK to use THF (tetrahydrofuran) on my HPLC?

Well, it might be. In the early days of HPLC, THF was commonly used as a mobile phase solvent. It has eluting strength similar to acetonitrile, but just slightly stronger. Since it technically is an ether and is very miscible with water, it is sometimes useful with reverse phase HPLC. It also provides additional options […]