Posts Tagged ‘liquid chromatography’

Can I use a 100% aqueous mobile phase with my LC column?

You might be asking this because you have read that not all columns are OK to use with highly aqueous content (>95%) in the mobile phase.  Or maybe the caption caught your eye because you have recently encountered some difficulty with using highly aqueous mobile phase. There is a phenomenon called “phase dewetting” that can […]

How Do I Scale Up to a Prep LC Column?

  As mentioned in my previous post What is Different about Prep LC?, we ask that you start by developing your method on an analytical scale column. Once you have accomplished this, and wish to start using prep scale HPLC for purification, you will need to determine what size preparative scale (“prep”) column would be […]