Posts Tagged ‘phase collapse’

Can I use a 100% aqueous mobile phase with my LC column?

You might be asking this because you have read that not all columns are OK to use with highly aqueous content (>95%) in the mobile phase.  Or maybe the caption caught your eye because you have recently encountered some difficulty with using highly aqueous mobile phase. There is a phenomenon called “phase dewetting” that can […]

More Technical Service “Red Flags” – LC

This post is the second of its kind pertaining to LC analysis, all of which are an extension of a series pertaining to “Red Flags” for GC analysis. These are situations and symptoms that tell us in the Tech Service group that something is just not right. As we discussed in the first post, Technical […]

A C18 is a C18, right?

    Sorry, that actually is not true. Although many methods only list the USP code L1 for all C18 HPLC columns, there are many different varieties of C18 columns available from Restek. Here are some of the ways they may differ:   Differences in Silica Depending on the silica particle that the C18 phase […]

[4]Troubleshooting HPLC- Fronting Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we have looked at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. Any time you have difficulty with poor peak shape, it is also […]