Archive for the ‘Optimizing Applications’ Category

Modifying QuEChERS for complicated matrices- Soil and Sediment

In previous blog posts for this series on QuEChERS, we discussed QuECHERS dSPE Selection, Modifying QuECHERS..-Dry Samples, Modifying QuEChERS..-High Fat Samples, and Modifying QuEChERS..-High Sugar & Starch. Once again, I suggest using the following documents from the official QuEChERS website (, maintained by CVUA Stuttgart: For Extraction (Stage 1): For dSPE Cleanup (Stage 2): […]

FDA Warns Hand Sanitizers May Contain 1-Propanol

The Food and Drug Administration (FDA) has expanded their recall of hand sanitizers and found more chemical contaminants to include 1-propanol (n-propanol cas# 71-23-8). 2-propanol (isopropanol cas# 67-63-0) and Ethanol (cas# 64-17-5) are the two most widely used active ingredients in hand sanitizers. With this latest addition to the list, the main concern for exposure […]

Not Every Matrix-Matched Calibration Is Made Equal – Case of Spinach

Matrix-matched calibrations are a popular option for complex matrices, such as food commodities. When it is employed, one can assume that the majority of matrix effects will be accounted for and there won’t be much difference between calibration standards and the tested samples. But are all matrix-matched calibrations equal? In the case of QuEChERS extraction, […]

Derivatization techniques for free fatty acids by GC

Anyone who has tried to analyze free fatty acids by GC realizes they have to derivatize these compounds. The inherently low volatility of free fatty acids and the carboxylic acid (-COOH) interaction with the siloxanes in the stationary phase. If not addressed, the first problem leads to late-eluting peaks and the latter results in peak […]

FDA Issues Second Warning on Methanol Based Hand Sanitizers

The U.S. Food and Drug Administration has placed methanol containing hand sanitizers on an import alert (1). These products do not list this ingredient and some are incorrectly labeled “FDA approved.” Currently, 87 hand sanitizers have been found to contain methanol (2). Methanol (methyl alcohol Cas# 67-56-1), known as wood alcohol, is commonly found in […]

Thermo Trace 1310 Inlet Temperature Profile vs Agilent 7890 for Split/Splitless Injectors

Several years ago, my colleague Scott Grossman wrote an excellent article entitled “It’s a Matter of Degrees, but Do Degrees Really Matter?”  He measured the temperature profile across various Agilent inlets, demonstrating different gradients in temperature exist across inlets, depending on the type of inlet and even among the same inlet type.  One consistent finding […]

Hydrocarbon Ranges of Natural Gas and Petroleum Products

Sometimes customers need to analyze natural gas and petroleum products, whether it is to confirm the purity of the product by looking for the impurities which may be present, or for an environmental reason such as a spill or leak.  In order to choose the correct GC column and reference standards, one needs to know […]

Modifying QuEChERS for complicated matrices- High Fat Samples

This post is part of a series on QuEChERS. Here are links to the previous two posts, in case you may wish to catch up before reading this one: QuEChERS dSPE selection-which one is best? Modifying QuECHERS for complicated matrices- Dry Samples (See Jana’s post here for further details on dry samples: QuECHERS approach optimization […]

Optimizing Splitless Injections: Initial Oven Temperature and Solvent Polarity

Beyond optimizing the inlet parameters of temperature and splitless valve time, initial oven temperature also plays an important role for splitless injections. When a liquid sample is injected into a GC, the first goal is to vaporize the sample within the inlet and transfer it to the column. As you know, this sample volatilization and […]

Optimizing Splitless Injections: Splitless Purge Valve Time

In my previous blog, I discussed optimizing inlet temperature for splitless injections.  Today I would like to discuss another critical parameter for splitless injections: splitless purge valve time.  The key feature of a splitless injection is that all the carrier gas flow is directed to the column and the splitless valve is closed during injection.  […]