How Dirty Are You? Part 3…Gloves…The Question


This third installment of How Dirty Are You? is all about gloves. Outside of solvent compatibility, there is a lot of room for personal preference regarding the type of lab gloves people can use.

I found 5 different gloves in our lab. The bright green gloves and yellow gloves are the two types most often used by my lab mates. I use the bright green gloves. I work with QuEChERS extracts all the time so I was most interested in background resulting from gloves contacting acetonitrile. I have already investigated the bright green gloves when I was tracking down some peaks in my process blank for a QuEChERS experiment. I was seeing some repeating peaks which were identified as alkanes via GC-TOFMS. Through some further investigation, I was able to confirm that these alkane peaks were from my favorite bright green gloves. That got me thinking about contamination peaks from other gloves…

What We Did


We tested the background generated by the 5 glove types found in our lab by soaking a small piece of glove in 1 mL of acetonitrile for 30 minutes. The samples were tested via GC-TOFMS after the 30 minutes. For relative intensity, a dashed line at the peak height of a 2 ppm PAH standard is drawn on the chromatograms. But you will not see the results until my next blog!



1. Which gloves (see below) gave the worst background?


Bright Green Gloves

Baby Blue Gloves

Yellow latex Gloves

Green Gloves

Royal Blue Gloves

2. What common additive was found in multiple gloves tested?




UV Stabilizers


8 Responses to “How Dirty Are You? Part 3…Gloves…The Question”

  1. Robert says:

    1. Green Gloves.

    2. UV Stabilizers.

  2. Thank you for posting your answers Robert!

  3. Becca Stevens says:

    1) yellow latex gloves


  4. 1) Royal Blue
    2) UV Stabilizers

    Of course I would not use any glove other than a Butyl glove when handling acetonitrile (methyl cyanide) in a fume hood, as nitrile gloves typically have a 30 minute breakthrough for acetonitrile.

  5. Stefan Thomas says:

    1. Latex
    2. UV stabilizers

  6. Hi Stefan,

    take a look at the answers here

    thank you for participating!

  7. It’s great to see some study on this type of work. Soaking gloves sections in ACN for 30min is very tough test, we’ve seen gloves that were just splashed with solvents contaminate an entire bottle before. Latex seems to be especially easy to contaminate with, so I’m going with Latex gloves. And UV stabilizers are soluble in ACN.

  8. make sure the check out the answers

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