One Stop Shop for Essential Oils Chromatograms

In the past few months, we have collected a variety of chromatograms using two different column phases, and three different column dimension formats. The peak identifications were performed using some standards but mostly from the NIST MS EI spectra library, 2005 edition. Let us know what you think in the comments below.

Citronella Java








Tea Tree




10 Responses to “One Stop Shop for Essential Oils Chromatograms”

  1. Peggy Biser says:

    I teach chemistry at Frostburg State Univeristy. I have a research student that is interested in spearmint. Is there a possibility that you might have a spearmint chromatogram? I see that you have peppermint. Would you suggest that the conditions under which you ran peppermint would be a good starting point for spearmint?

    Thank you for this resource. Best to all in this difficult time.

  2. Jana Rousova says:

    Hello Peggy!
    Thank you for the excellent question! Yes, I believe that using the same conditions for spearmint as we used for peppermint would be a good option. I’d personally use the Rtx-Wax because it offers better separation between menthone and isomethone (in the chromatogram as menthone 1 and 2).

  3. Kepher Kuchana Kateu says:

    Dear Sur/Madam
    Good morning
    I am the head of Government Analytical Laboratory in Uganda
    I am interested in getting the analytical protocol of analysing the major essential oils and the chromatograms of Eucalyptus, peppermint, rosemary, lemon (citrus)
    Thank you

  4. Vanessa says:

    Hi, I am working in business about citrus essential oil, we always have problems with the p-Cymene compound and D-Limonene because we can not separate completely between them. Could you help me with that o recommended something?


  5. Amazing work! Thanks for making this available, it’s very useful.
    I’m helping my wife (Family doctor in the public health system here in Spain) in her investigation about the effect of essential oils on the human body. I’ve been going through your documentation but couldn’t find anything related to the samples you used, could you provide some additional information, please? Were those chemotype? Thanks a lot in advance! :)
    Best regards from Spain

  6. Shareef Alhashemi says:

    why dont have chromatography for Luban Oil (Fragrance essential Oil )

  7. Jana Rousova says:

    Thank you for the suggestion, we’ll consider Luban (frankincense) oil for our future work.
    Are choice was based on our previous work and oil availability.
    Best Regards,

  8. Jana Rousova says:

    Hello Vanessa,
    From your question I assume you’re using either Rxi-5 or Rxi-5Sil. We have a proEZGC library for Rxi-5MS with terpenes, including p-Cymene and D-Limonene, so you can try to adjust your method to get the best separation.
    Alternatively, have you tried using wax column? Rtx-Wax does great job separating those two terpenes: (peak 5 and 8), (peak 8 and 11) and (peak 5 and 9).
    Please, don’t hesitate to contact me further either here or via email.

    Best regards,

  9. Jana Rousova says:

    Hello Angel,
    Thank you for your kind works! We obtained our samples either from Millipore Sigma (citronella, lemon, rosemary, bergamot and patchouli) or from a local grocery store. As far as I know, these were not chemotypes. The samples themselves were simply diluted in acetone. If you’d like more information, please don’t hesitate to reply or send me an email.

  10. Jana Rousova says:

    We followed the same simple protocol for all the oils: we dissolved them in acetone (5% by volume) and analyzed by GC, injecting in 100:1 split. Each of the individual chromatograms have all the pertinent conditions.
    If you need more information, please let me know via email (
    Best regards,

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