You might be asking this because you have read that not all columns are OK to use with highly aqueous content (>95%) in the mobile phase. Or maybe the caption caught your eye because you have recently encountered some difficulty with using highly aqueous mobile phase. There is a phenomenon called “phase dewetting” that can […]
Posts Tagged ‘aqueous mobile phase’
[4]Troubleshooting HPLC- Fronting Peaks
Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error. In this brief series of posts, we have looked at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. Any time you have difficulty with poor peak shape, it is also […]
