Posts Tagged ‘CAC’

European Food Safety Authority (EFSA) PAH4 Separated on Rxi-35Sil MS – The Coalition Against Coelution (CAC) Continues Its Dominance over Polycyclic Aromatic Hydrocarbons (PAHs)

The Coalition Against Coelution (CAC) is putting together all the pieces for you now on the most important PAHs according to the European Food Safety Authority’s Scientific Opinion of the Panel on Contaminants in the Food Chain for Polycyclic Aromatic Hydrocarbons. The PAH4, consisting of benzo[a]pyrene, benz[a]anthracene, chrysene, and benzo[b]fluoranthene, are representative of the carcinogenic […]

Join the Coalition and Strike a Blow for Resolution!

The Coalition Against Coelution (CAC) stands for One Compound, One Peak, regardless of class. Speak out about the critical separations that matter most to you, and the tools you use to overthrow the oppressor of coelution. We’ve already looked at GC column parameters, serially-connected LC columns, and a software tool (hey Jack, what about EZ […]

Chromatography and “Color Writing” in Prague – Coalition Against Coelution Seen at Lennon Wall and RAFA

The word chromatography, or “color writing”, originated from Mikhail Tsvet, and was first described in his separations of plant pigments (e.g. chlorophyll, carotenes, xanthophylls) on a calcium carbonate column with organic solvent elution. Here in Prague for the 5th International Symposium on Recent Advances in Food Analysis (RAFA 2011), my colleague, Julie Kowalski, and I […]

GC Method Translation Software Used by CAC to Promote Faster Analysis Times While Maintaining Critical PAH Separations – Benzo[a]Pyrene

UPDATE:  The Coalition Against Coelution strikes another blow for chromatographic separation by offering a new version of the Method Translator and Flow Calculator.  You’ll see a brilliant series of posts soon, starting with Using the Restek EZGC Method Translator and Flow Calculator to Support Shoot-and-Dilute GC Method Development – Going from GC-ECD to GC-MS, that demonstrate […]

Comparing Rxi-17Sil MS to Rxi-35Sil MS for Critical PAH Separations

The Coalition Against Coelution charges ahead with additional GC PAH separations and now compares Rxi-17Sil MS (50% diphenyl-type with a silarylene backbone) and Rxi-35Sil MS (35% diphenyl-type with a silarylene backbone).  Note that we do a bit better with triphenylene and chrysene on the 35Sil MS, but give up a bit of resolution on the […]

The Coalition Against Coelution (CAC) and GC Method Translation for PAHs

Previously the CAC showed separations for PAHs triphenylene and chrysene, and the benzo fluoranthenes, on a 60m x 0.25mm x 0.10µm Rxi-17Sil MS (thank you Ron Stricek, once again!).  In this blog, we shorten the run time by a factor of 1.6 using column miniaturization (40m x 0.18mm x 0.07µm Rxi-17Sil MS – kindly made […]

CAC Accepts Benzo Fluoranthenes Separation Challenge – Uses Rxi-17Sil MS

The CAC continues its work with the 60m x 0.25mm x 0.10µm Rxi-17Sil MS, after previously showing separation of triphenylene and chrysene, by demonstrating baseline resolution of benzo fluoranthenes, including b, k, j, and a.  This is important because the European Food Safety Authority has recommended monitoring of carcinogenic PAHs benzo[b]fluoranthene and benzo[k]fluoranthene.  To determine […]

The Coalition Against Coelution (CAC) Crafts Chrysene Chromatogram from Coal Tar

Here is a chrysene and triphenylene separation in a “real world” coal tar Standard Reference Material from NIST (1957a – Thanks Michele!) on a 60m x 0.25mm x 0.10µm Rxi-17Sil MS. Although you have to “drop perpendiculars” to integrate them, a good quantitative estimate can still be made for chrysene, the more important of these […]

Join the Restek CAC and Separate Triphenylene and Chrysene and other PAHs, and Many Other Compounds

Restek just founded the Coalition Against Coelution (CAC) based around their highly selective stationary phases for GC and LC.  If you stroll around on the website and the blog, you’ll find the CAC manifesto in action, whether it is the Rtx-Dioxin2 GC column, with its TCDD and TCDF specificity; the Rtx-CLPesticides GC columns, which are […]