Posts Tagged ‘dead volume’

[3]Troubleshooting HPLC- Tailing Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. If you are having difficulty with tailing, it is likely that you […]

Do reverse phase HPLC columns really need conditioning?

  Well, it’s partly a matter of semantics sometimes, but there is usually nothing drastic that you need to do to get your HPLC column going if it is new.  There is no baking at high temperatures involved like you might do for GC or that sort of thing. You do, however, need to equilibrate […]