Posts Tagged ‘flow rate’

What is the pressure limit for my LC column?

This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ROC LC columns, the maximum operating pressure is 400 […]

How Do I Scale Up to a Prep LC Column?

  As mentioned in my previous post What is Different about Prep LC?, we ask that you start by developing your method on an analytical scale column. Once you have accomplished this, and wish to start using prep scale HPLC for purification, you will need to determine what size preparative scale (“prep”) column would be […]

Measuring Capillary Column Flow: “To ProFLOW or not to ProFLOW, that is the question”

Occasionally the Restek Technical Service group gets a request from a customer for information on how to measure the carrier gas flow from their capillary column with the Restek ProFLOW 6000 Electronic Flowmeter.     Although this electronic flowmeter is designed to measure volumetric flow for gases across a range of 0.5–500 mL/minute, there are […]

Connecting a Restek ProFLOW 6000 Electronic Flowmeter to an Agilent Split Vent or Septum Purge

The Restek ProFLOW 6000 Electronic Flowmeter is a very useful tool for the measurement of any type of gas commonly used in chromatography. The tubing coming out of the ProFLOW 6000 is made of a flexible elastomer about 28” long with a ¼” OD and a 1/8” ID. We occasionally get questions about how to […]

Building up pressure on HPLC?

We’ve all experienced a pressure error on HPLC at some time or another. Here are my best suggestions on handling them. Establish ground rules-This will help to define the problem and distinguish if there really is one. 1.     Check your programmed max pressure limit. Be aware of what the operating limit is for your system and […]