Posts Tagged ‘mobile phase’

What can I use to filter my mobile phase?

If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed […]

What can I use as a cap for my mobile phase bottles?

Our choices for mobile phase bottle tops are based primarily on two different products. The first product is the Hub-Cap Bottle Top, catalog #26541. This is designed to fit a 4-Liter solvent bottle. :     The body (also called insert) of the Hub-Cap Top is cap is made of inert PTFE and has three […]

Calculating pH

I’m guessing that most of us that work in the lab would find it more convenient to measure a pH rather than to calculate a theoretical value.  But, at times, it can be very useful to make that calculation and get an idea of what to expect.  As far as chromatography is concerned, all columns […]

[3]Troubleshooting HPLC- Tailing Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. If you are having difficulty with tailing, it is likely that you […]

I have an Ultra Quat column and I know how to use it….

Are those words you wish you could say? Most recently, we seem to encounter more customers asking for advice in preparing a mobile phase for their Diquat and Paraquat analysis (related to EPA Method 549). There are a couple of different scenarios for this analysis. Please note that the procedures described here are intended for […]

Which LC Phase Do I Need?

So you’re thinking about doing HPLC (or UHPLC) and don’t have a method? You’ve never even seen a method for anything remotely similar. You might have even searched our HPLC chromatogram database for the application and haven’t found anything.  (After all, that’s the quickest and easiest way to get some ideas.) What to do… Let’s […]