Posts Tagged ‘pH’

Calculating pH

I’m guessing that most of us that work in the lab would find it more convenient to measure a pH rather than to calculate a theoretical value.  But, at times, it can be very useful to make that calculation and get an idea of what to expect.  As far as chromatography is concerned, all columns […]

How much buffer to weigh and what’s the best way to prepare my buffered mobile phase?

Occasionally we get this question and sometimes folks do need help with the calculations. We discussed in a previous post what a buffer is composed of. Click on this link if you need to review it: When should you use a buffer for HPLC, how does it work and which one to use? For a […]

Live Long and Prosper-How to make your HPLC columns last longer

We frequently get questions about how long an HPLC or UHPLC column should last. To borrow a phrase from my colleagues, “Your mileage may vary.”  Columns are like cars, so their lifetime depends how they are used and how well they are maintained. That being said, some phases are known to be more rugged than […]