Posts Tagged ‘SPP’

Mass Overload on Superficially Porous Particles

The benefits of superficially porous particles are without question.  Who wouldn’t want to perform faster separations without the need for expensive UHPLC instrumentation?  It sounds too good to be true.  There must be some drawback – right? The solid, impermeable core present in Raptor™ particles increases column efficiency by decreasing the diffusion path at the […]

How to decrease run time for reversed phase LC

There are several ways to accomplish this, although it boils down to roughly 4 possibilities. Use a shorter column length. This may be appropriate if you have plenty of retention for your analyte(s), if you only have one or a few analytes AND if you do not have interference peaks in your chromatogram. It does, […]

Should I use a 2.7 or 5 µm Raptor™ column?

“When you come to a fork in the road, take it.” Maybe that advice worked for Yogi Berra, but does not help much in decisions about your lab equipment. If you are not certain whether you should use a Raptor™ column, please click on the title below to read my previous blog post before proceeding. […]

What is SPP and when should I use a Raptor column?

While this sounds like a very broad question, I’m sure it is crossing some of your minds. To start out, SPP stands for superficially porous particles. It has also been called “core-shell”.   If you are a veteran in the world of HPLC, this term is old hat for you. If you are new to the […]

A C18 is a C18, right?

    Sorry, that actually is not true. Although many methods only list the USP code L1 for all C18 HPLC columns, there are many different varieties of C18 columns available from Restek. Here are some of the ways they may differ:   Differences in Silica Depending on the silica particle that the C18 phase […]